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MMP-8 Antibody (JE59-38)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32601

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32601

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE59-38

Concentration

1 mg/ml

Product Summary for MMP-8 Antibody (JE59-38)

Immunogen

Recombinant protein within Human MMP-8 aa 101-200 / 467. (Uniprot: P22894)

Localization

Cytoplasmic granule, Secreted, extracellular space, extracellular matrix.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMP-8 Antibody (JE59-38)

MMP-8 Antibody (JE59-38)

Immunohistochemistry: MMP-8 Antibody (JE59-38) [NBP3-32601]

Immunohistochemistry: MMP-8 Antibody (JE59-38) [NBP3-32601] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-MMP-8 antibody (NBP3-32601) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32601) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for MMP-8 Antibody (JE59-38)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-8

MMP8 is a Metallopeptidase M10A type protease. MMP8 is a Metallopeptidase M10A type protease. It is synthesized and stored intracellularly in specific granules in neutrophils. MMP8 shows a preference for degrading type I collagen.

Long Name

Matrix Metalloproteinase 8

Alternate Names

Collagenase 2, MMP8

Gene Symbol

MMP8

Additional MMP-8 Products

Product Documents for MMP-8 Antibody (JE59-38)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MMP-8 Antibody (JE59-38)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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