MMP-8 Antibody (MM0023-7A11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60989

Novus Biologicals, part of Bio-Techne
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NB110-60989
NB110-60989-0.025mg
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Clone # MM0023-7A11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MMP-8 Antibodies »

Product Specifications

Immunogen

Human recombinant MMP-8

Reactivity Notes

This antibody is reactive against human. Not yet tested in other species.

Specificity

This antibody recognizes human MMP-8 in western blot without cross reactivity with other MMPs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Scientific Data Images for MMP-8 Antibody (MM0023-7A11) - Azide and BSA Free

Immunohistochemistry-Paraffin: MMP-8 Antibody (MM0023-7A11) - Azide and BSA Free [NB110-60989]

Immunohistochemistry-Paraffin: MMP-8 Antibody (MM0023-7A11) - Azide and BSA Free [NB110-60989]

Immunohistochemistry-Paraffin: MMP8 Antibody (MM0023-7A11) [NB110-60989] - Human placenta (formalin fixed and paraffin embedded) section is subjected to IHC.

Applications for MMP-8 Antibody (MM0023-7A11) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:400-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-8

Matrix Metalloproteinase 8 (MMP8) is also known as neutrophil collagenase and collagenase 2. MMP8 degrades fibrillar collagens types I, II, III, aggrecan, serpins and alpha 2 macroglobulin. All collagenases cleave fibrillar collagens at one specific site resulting in generation of N terminal three quarter and C terminal one quarter fragments, which then denature to gelatin at body temperature. The substrate specificity of collagenases is variable: MMP1 degrades type III collagen more efficiently than type I or type II collagen, whereas MMP8 is more potent in degrading type I collagen than type III or type II collagen. MMP13, in turn degrades type II collagen 6 fold more efficiently than type I and type II collagens and displays almost 50 fold stronger gelatinolytic activity than MMP1 and MMP8. MMP8 is very similar to MMP1, sharing 57 % amino acid identity. Most cell types do not produce MMP8. Until recently, it was thought that MMP8 was produced exclusively by neutrophils, but it has also been detected in other cell types including arthritic chondrocytes and gingival fibroblasts. The human MMP8 gene has the chromosomal location of 11q22.2-22.3. MMP8 is heavily glycosylated, and the zymogen has a mass of 85 Kd. The zymogen is quickly activated to the 64 Kd form, and this breaks down to a cascade of active forms.

Long Name

Matrix Metalloproteinase 8

Alternate Names

Collagenase 2, MMP8

Entrez Gene IDs

4317 (Human)

Gene Symbol

MMP8

Additional MMP-8 Products

Product Documents for MMP-8 Antibody (MM0023-7A11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MMP-8 Antibody (MM0023-7A11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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