MMP-9 Antibody (4A3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80855
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
CyTOF-ready, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
CyTOF-ready, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4A3
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide from the N-terminus of human MMP9 [Swiss-Prot# P14780]
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 30772382).
Localization
Secreted > extracellular space > extracellular matrix
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for MMP-9 Antibody (4A3) - Azide and BSA Free
Western Blot: MMP-9 Antibody (4A3)Azide and BSA Free [NBP2-80855]
Western Blot: MMP-9 Antibody (4A3) - Azide and BSA Free [NBP2-80855] - Identification of gelatinases by Western blot analysis. A pool of sera from 10 patients with inactive RR-MS was used. For 2-DZ, pooled sera were concentrated 10-fold and 40 ug of total proteins were applied (left panels). For Western blot analysis pooled sera were concentrated 40-fold and 160 ug of total proteins were subjected to 2-DE (right panels). 2-DE gels were cut and the corresponding nitrocellulose membranes were treated with anti-NGAL Ab (A'), anti-MMP-2 Ab (B') and Anti-MMP-9 (4A3) Ab, recognizing the 65 kD and the 82 kD activated forms (C'); Gel C show the region with isoelectric point between pH 4-5.5 and molecular mass between 85 and 55 kD, analysed by Western blot in C', respectively. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY license. Image from the standard format of this antibody.Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) - Azide and BSA Free [NBP2-80855]
Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) - Azide and BSA Free [NBP2-80855] - Analysis of a FFPE section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled secondary antibody and DAB reagent followed by hematoxylin counterstaining. This antibody clone generated an extraWestern Blot: MMP-9 Antibody (4A3)Azide and BSA Free [NBP2-80855]
Western Blot: MMP-9 Antibody (4A3) - Azide and BSA Free [NBP2-80855] - 1.5 ul of serum from healthy control (HC) or from a patient with inactive RR-MS loaded on a 7.5% polyacrylamide gel copolymerized with 0.1% (w/v) gelatin (1-DZ). 2 ul of fivefold concentrated (Vivaspin 500, MWCO 50,000; GE Healthcare) serum samples (the same as in 1-DZ) separated on a 7.5% polyacrylamide gel without gelatin (1-DE), transferred on nitrocellulose membranes & incubated with the following primary antibodies: Anti-NGAL (5G5) at 2.0 ug/ml; Anti-MMP-9 (Ab-8) (IA5) at 2.66 ug/ml; Anti-MMP-2 (Ab-4) (75-7F7) at 1.0 ug/ml & Anti-MMP-9 Ab (4A3) at 2.0 ug/ml. Image collected & cropped by CiteAb from the following publication (null), licensed under a CC-BY license. Image from the st&ard format of this antibody.Applications for MMP-9 Antibody (4A3) - Azide and BSA Free
Application
Recommended Usage
CyTOF-ready
reported by customer review
ELISA
1:100 - 1:2000
Immunohistochemistry
1:100-1:200
Immunohistochemistry-Paraffin
1:100-1:200
Western Blot
1:500-1:1000
Application Notes
In Western blot a band can be seen at 82 or 63 kDa, representing the active forms of MMP9. In IHC, cytoplasmic and extracellular staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NBP2-80855 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine (pH 7.5), 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MMP-9
Long Name
Matrix Metalloproteinase 9
Alternate Names
CLG4B, Gelatinase B, GELB, MANDP2, MMP9
Gene Symbol
MMP9
Additional MMP-9 Products
Product Documents for MMP-9 Antibody (4A3) - Azide and BSA Free
Product Specific Notices for MMP-9 Antibody (4A3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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