MMP-9 Antibody (5G3) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22181
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Cited:
ELISA, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 5G3
Format
BSA Free
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Purified recombinant fragment of human MMP-9 expressed in E. Coli.
Localization
Cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MMP-9 Antibody (5G3) - BSA Free
Western Blot: MMP-9 Antibody (5G3)BSA Free [NBP2-22181]
Western Blot: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis using MMP9 mAb against human MMP9 (AA: 238-465) recombinant protein. (Expected MW is 50.6 kDa)Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody (5G3) - BSA Free [NBP2-22181]
Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of NIH/3T3 cells using MMP9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Immunohistochemistry-Paraffin: MMP-9 Antibody (5G3) - BSA Free [NBP2-22181]
Immunohistochemistry-Paraffin: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of tissues using MMP9 mouse mAb with DAB staining.Applications for MMP-9 Antibody (5G3) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Format
BSA Free
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MMP-9
Long Name
Matrix Metalloproteinase 9
Alternate Names
CLG4B, Gelatinase B, GELB, MANDP2, MMP9
Gene Symbol
MMP9
Additional MMP-9 Products
Product Documents for MMP-9 Antibody (5G3) - BSA Free
Product Specific Notices for MMP-9 Antibody (5G3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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