Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S51-82

Concentration

1 mg/ml

View all MMP-9 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1-708 (full length) of rat MMP9

Localization

Extracellular Matrix

Specificity

Detecs ~92kDa and ~82kDa (pro and active forms).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for MMP-9 Antibody (S51-82)

Western Blot: MMP-9 Antibody (S51-82) [NBP2-59699]

Western Blot: MMP-9 Antibody (S51-82) [NBP2-59699]

Western Blot: MMP-9 Antibody (S51-82) [NBP2-59699] - Western Blot analysis of Rat Brain showing detection of ~92 kDa and ~82 kDa (pro and active) MMP-9 protein using Mouse Anti-MMP-9 Monoclonal Antibody, Clone S51-82 (NBP2-59699). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MMP-9 Monoclonal Antibody (NBP2-59699) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~92 kDa and ~82 kDa (pro and active).
Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699]

Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699]

Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MMP-9 Monoclonal Antibody, Clone S51-82 (NBP2-59699). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-MMP-9 Monoclonal Antibody (NBP2-59699) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) MMP-9 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699]

Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699]

Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699] - Analysis using Mouse Anti-MMP9 Monoclonal Antibody, Clone S51-82 . Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) MMP9 Antibody (D) Composite.
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Applications for MMP-9 Antibody (S51-82)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of this antibody was sufficient for detection of MMP9 in 20 ug of COS-1 cells (lysate) transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-9

MMP9, otherwise known as matrix metalloproteinase 9, is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction and tissue remodeling, as well as in disease processes like arthritis and metastasis (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for MMP-9 Antibody (S51-82)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MMP-9 Antibody (S51-82)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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