Mouse 4-1BB Ligand/TNFSF9 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1246
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Arg104-Glu309
Accession # P41274
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse 4-1BB Ligand/TNFSF9 Antibody
Detection of 4-1BB Ligand/TNFSF9 in Raw264.7 cells by Flow Cytometry.
Raw264.7 cells were stained with Rat Anti-Mouse 4-1BB Ligand/TNFSF9 Monoclonal Antibody (Catalog # MAB1246, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse 4-1BB Ligand/TNFSF9 Antibody
CyTOF-ready
Flow Cytometry
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Western Blot
Sample: Recombinant Mouse 4-1BB Ligand/TNFSF9 (Catalog # 1246-4L)
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 4-1BB Ligand/TNFSF9
4-1BB ligand (4-1BBL) is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF9. Mouse 4-1BBL cDNA encodes a 309 amino acid residues (aa) protein with an 82 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C-terminal extracellular domain. The extracellular domain of 4-1BBL has a tertiary structure similar to that of other TNFSF members, but shares only low aa sequence homology (14‑16%). Murine 4-1BBL shares 36% aa sequence identity with its human counterpart (1, 2). 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines (3). A soluble 4-1BBL is released from the cell surface following cellular activation via proteolytic cleavage by one or more sheddases (4). By analogy to other TNFSF ligands, both the soluble and transmembrane 4-1BBL are expected to exist as non-covalent homotrimers. 4-1BBL binds 4-1BB, a TNF receptor superfamily member, TNFRSF9, which is also known as CD137 and ILA (induced by lymphocyte activation). 4-1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils. In response to 4-1BBL binding, 4-1BB transduces a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In dendritic cells, 4-1BB is a DC-activating molecule that enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules (3).
References
- Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631.
- Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
- Kwon, B., H.W. Lee and B.S Kwon (2002) TRENDS in Immunology 23:378.
- Salih, H.R. et al. (2001) J. Immunol. 167:4059.
Alternate Names
Gene Symbol
UniProt
Additional 4-1BB Ligand/TNFSF9 Products
Product Documents for Mouse 4-1BB Ligand/TNFSF9 Antibody
Product Specific Notices for Mouse 4-1BB Ligand/TNFSF9 Antibody
For research use only