Mouse A33 Antibody

Name: Mouse A33 Antibody
Brand: R&D Systems
SKU: AF2756

R&D Systems, part of Bio-Techne | Catalog # AF2756

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Product Datasheet / COA / SDS

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all A33 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse A33
Leu22-Ile235
Accession # Q9JKA5

Specificity

Detects mouse A33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human A33 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse A33 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse A33

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A33

Mouse A33, also known as Gpa33, is a 48 kDa type I transmembrane protein belonging to the CTX (cortical thymocyte marker in Xenopus) family of cell-adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular region of A33 contains a V-type and a C2-type Ig-like domain. A33 is believed to be involved in cell-cell adhesion, either between epithelium or between epithelium and T cells. The extracellular domain of mouse A33 shares 87% and 71% amino acid sequence identity with the extracellular region of rat and human A33, respectively.

Long Name

Cell Surface A33 Antigen

Alternate Names

GPA33

Entrez Gene IDs

10223 (Human); 59290 (Mouse)

Gene Symbol