Mouse alpha-Fetoprotein/AFP Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF5369
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse alpha‑Fetoprotein/AFP
Lys19-Val605
Accession # P02772
Lys19-Val605
Accession # P02772
Specificity
Detects mouse alpha‑Fetoprotein/AFP in Western blots. In Western blots, approximately 10% cross-reactivity with human alpha-Fetoprotein is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse alpha-Fetoprotein/AFP Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse alpha‑Fetoprotein/AFP
Sample: Recombinant Mouse alpha‑Fetoprotein/AFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Fetoprotein/AFP
AFP ( alpha-Fetoprotein)is a 69-73 kDa member of the ALB/AFP/VDB family of proteins. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature mouse AFP is 587 amino acids (aa) in length. It contains three albumin domains (aa 20-201, 208-393 and 400-591), plus 15 intrachain disulfide bonds. Rat AFP contains an alternate start site at Met287 that may generate an intracellular 37 kDa form. Mouse AFP also shows an analogous site at Met281 that may produce an equivalent isoform. Over aa 19-605, mouse AFP shares 83% and 66% aa identity with rat and human AFP, respectively.
Alternate Names
AFP, DSCAM2
Gene Symbol
AFP
UniProt
Additional alpha-Fetoprotein/AFP Products
Product Documents for Mouse alpha-Fetoprotein/AFP Biotinylated Antibody
Product Specific Notices for Mouse alpha-Fetoprotein/AFP Biotinylated Antibody
For research use only
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