Mouse ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3558
Key Product Details
Species Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ASAH2/N‑acylsphingosine Amidohydrolase-2
Thr34-Thr756
Accession # Q9JHE3
Thr34-Thr756
Accession # Q9JHE3
Specificity
Detects mouse ASAH2/N‑acylsphingosine Amidohydrolase-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human ASAH2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse ASAH2/N-acylsphingosine Amidohydrolase-2 (Catalog # 3558-AH), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse ASAH2/N-acylsphingosine Amidohydrolase-2 (Catalog # 3558-AH), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse ASAH2/N-acylsphingosine Amidohydrolase-2 (Catalog # 3558-AH)
Sample: Recombinant Mouse ASAH2/N-acylsphingosine Amidohydrolase-2 (Catalog # 3558-AH)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ASAH2/N-acylsphingosine Amidohydrolase-2
The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, also known as neutral ceramidase. Neutral ceramidase is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein (1). The enzyme is abundant in the brush border membranes of the intestine, but is also expressed in tissues such as kidney, brain and liver (2, 3). A major physiological function of neutral ceramidase is the metabolism of dietary sphingolipids, but the enzyme may also be involved in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate (3).
References
- Tani, M. et al. (2003) J. Biol. Chem. 278:10523.
- Kono, M. et al. (2006) J. Biol. Chem. 281:7324.
- Mitsutake, S. et al. (2001) J. Biol. Chem. 276:26249.
Long Name
Neutral Ceramidase
Alternate Names
Nacylsphingosine Amidohydrolase2
Gene Symbol
ASAH2
UniProt
Additional ASAH2/N-acylsphingosine Amidohydrolase-2 Products
Product Documents for Mouse ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody
Product Specific Notices for Mouse ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody
For research use only
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