Mouse ASCL1/Mash1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2567
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse ASCL1/Mash1
His74-Asp173 (Gln160Glu)
Accession # Q53WV9
His74-Asp173 (Gln160Glu)
Accession # Q53WV9
Specificity
Detects mouse ASCL1/Mash1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse ASCL1/Mash1 Antibody
ASCL1/Mash1 in Mouse Embryo.
ASCL1/Mash1 was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c., cross-section through developing CNS) using Goat Anti-Mouse ASCL1/Mash1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2567) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Mouse ASCL1/Mash1 Antibody
Application
Recommended Usage
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c., cross-section through developing CNS)
Sample: Immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c., cross-section through developing CNS)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse ASCL1/Mash1
Sample: Recombinant Mouse ASCL1/Mash1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4 using AF2567 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ASCL1/Mash1
Long Name
Achaete Scute Complex-like 1
Alternate Names
ASCL1, ASH1, HASH1, Mash1
Gene Symbol
ASCL1
UniProt
Additional ASCL1/Mash1 Products
Product Documents for Mouse ASCL1/Mash1 Antibody
Product Specific Notices for Mouse ASCL1/Mash1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...