Mouse B7-H2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB158
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 599841
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7-H2
Glu47-Lys279
Accession # Q9JHJ8
Glu47-Lys279
Accession # Q9JHJ8
Specificity
Detects mouse B7-H2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-H2, rhB7-H3,rhB7-H3b, rhPD-L2, recombinant mouse (rm) B7-1, rmB7-2, rmB7-H1, rmB7-H3, rmB7-H4, rmPD-L2, or recombinant rat B7-2 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse B7-H2 Antibody
Detection of B7‑H2 in A20 Mouse Cell Line by Flow Cytometry.
A20 mouse B cell lymphoma cell line was stained with Mouse B7-H2 Monoclonal Antibody (Catalog # MAB158, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).
B7‑H2 in Mouse Splenocytes.
B7-H2 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB158) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse B7-H2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: A20 mouse B cell lymphoma cell line
Sample: A20 mouse B cell lymphoma cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB158 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-H2
B7-H2, also called B7RP-1 and ICOSL, is a 60 kDa member of the B7 family of immune costimulatory proteins. It is expressed on resting B cells, dendritic cells, monocytes, alveolar epithelial cells, and placental trophoblasts. B7-H2 interactions with ICOS promote lymphocyte differentiation and activation. Within the ECD, mouse B7-H2 shares 49% and 70% aa sequence identity with human and rat B7-H2, respectively.
Long Name
B7 Homolog 2
Alternate Names
B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG
Gene Symbol
ICOSLG
UniProt
Additional B7-H2 Products
Product Documents for Mouse B7-H2 Antibody
Product Specific Notices for Mouse B7-H2 Antibody
For research use only
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