Mouse BACH1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5777

R&D Systems, part of Bio-Techne
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AF5777
AF5777-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all BACH1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse BACH1
Ser133-Gly513
Accession # P97302

Specificity

Detects endogenous mouse BACH1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse BACH1 Antibody

Detection of Mouse BACH1 antibody by Western Blot.

Detection of Mouse BACH1 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse BACH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5777) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BACH1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse BACH1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BACH1

BACH1 (BTB and CNC homolog 1; also basic leucine zipper transcription factor 1) is a ubiquitously expressed member of the Bach family of transcription factors. Although its predicted MW is 82 kDa, it runs anomalously at 110 kDa in SDS-PAGE. BACH1 forms noncovalent homodimers, and heterodimers with Maf oncoproteins and p53-related proteins. It apparently serves as an architectural component for gene regulatory protein complexes. Mouse BACH1 is 739 amino acids (aa) in length. It contains a protein-interaction BTB domain (aa 24‑127), a DNA-binding motif (aa 565‑580), and a Leu-zipper domain (aa 588‑610). This molecule should not be confused with Bach1/Fancj/Brip1 helicase. Over aa 133‑513, mouse BACH1 shares 75% and 88% aa identity with human and rat BACH1, respectively.

Long Name

BTB and CNC homolog 1

Alternate Names

BTB, HA2303

Entrez Gene IDs

571 (Human); 12013 (Mouse); 304127 (Rat)

Gene Symbol

BACH1

UniProt

P97302

Additional BACH1 Products

Product Documents for Mouse BACH1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse BACH1 Antibody

For research use only

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