Key Product Details

Species Reactivity

Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 121808

View all BAFF/BLyS/TNFSF13B Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse BAFF
Ala127-Leu309
Accession # Q9WU72

Specificity

Detects mouse BAFF in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) 4-1BB Ligand, rmEDA, rmFas Ligand, rmOX40 Ligand, rmTNF-alpha, rmTRANCE, rmTWEAK, recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha1/ beta2, rhLymphotoxin alpha2/ beta1, rhTNF-alpha, rhTRAIL, rhVEGI, recombinant canine, cotton rat, equine, feline, porcine, or rat TNF-alpha.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse BAFF/BLyS/TNFSF13B APC-conjugated Antibody

Detection of BAFF/BLyS/TNFSF13B antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of BAFF/BLyS/TNFSF13B in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse BAFF/BLyS/TNFSF13B APC-conjugated Monoclonal Antibody (Catalog # IC1357A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Mouse BAFF/BLyS/TNFSF13B APC-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: BAFF/BLyS/TNFSF13B

BAFF (also known as TALL-1, BLyS, THANK) is a 34-36 kDa type II transmembrane glycoprotein belonging to the BAFF family of the TNF superfamily, and has been designated as TNF superfamily member 13B (TNFSF13B). Mouse BAFF is a 309 amino acid (aa) protein consisting of a 248 aa extracellular domain, a 21 aa transmembrane region and a 45 aa cytoplasmic tail. BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain. Mouse BAFF may be shed from the cell surface by proteolytic cleavage between R126 and Ala 127 to yield a soluble form of the protein detectable in serum. Within the TNF superfamily, BAFF shares the highest homology (48%) with APRIL, the only other member of the BAFF family of molecules. BAFF and APRIL both bind to BCMA and TACI, while BAFF also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which represents the principal BAFF receptor. All three receptors are type III transmembrane proteins that are expressed on B cells, monocytes, osteoclasts, activated T cells, adipocytes, keratinocytes and microglia. BAFF and APRIL can form active heteromers that bind TACI. BAFF is expressed by monocytes, osteoclasts, astrocytes, neutrophils, follicular T helper cells, dendritic cells and specialized fibroblasts. Its expression in resting monocytes is upregulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response. BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells. Recent reports also show a role for BAFF in osteoclast formation. Over aa 127-309, mouse BAFF shares 93 and 72% aa sequence identity with rat and human BAFF, respectively.

Long Name

B cell Activating Factor

Alternate Names

BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4

Entrez Gene IDs

10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)

Gene Symbol

TNFSF13B

UniProt

Q9WU72

Additional BAFF/BLyS/TNFSF13B Products

Product Documents for Mouse BAFF/BLyS/TNFSF13B APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse BAFF/BLyS/TNFSF13B APC-conjugated Antibody

For research use only

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