Mouse Bag-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF815
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Hamster, Transgenic Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Bag-1
Specificity
Detects mouse Bag-1 in Western blots. Does not react with human Bag-1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Bag-1 Antibody
Detection of Mouse Bag-1 by Western Blot.
Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.6 µg/mL of Goat Anti-Mouse Bag-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF815) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bag-1 at approximately 32 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Immunoprecipitation of Mouse Bag‑1.
Bag‑1 was immunoprecipitated from lysates (3 x 106cells) of CTLL‑2 mouse cytotoxic T cell line and A431 human epithelial carcinoma cell line following incubation with 5 µg Goat Anti-Mouse Bag‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF815) for 1 hour at 4 ºC. Bag‑1-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated Bag‑1 was detected by Western blot using 5 µg/mL Mouse Bag‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF815). The leftmost lane represents a control experiment where the antibody was incubated with no cell lysate. View our recommended buffer recipes for immunoprecipitation.Applications for Mouse Bag-1 Antibody
Application
Recommended Usage
Immunoprecipitation
2 µg/106 cells
Sample: CTLL-2 mouse cytotoxic T cell line, see our available Western blot detection antibodies
Sample: CTLL-2 mouse cytotoxic T cell line, see our available Western blot detection antibodies
Western Blot
5 µg/mL
Sample: Bag‑1 immunoprecipitated from lysates of CTLL‑2 mouse cytotoxic T cell line and A431 human epithelial carcinoma cell line
Sample: Bag‑1 immunoprecipitated from lysates of CTLL‑2 mouse cytotoxic T cell line and A431 human epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bag-1
Bag-1 (Bcl-2-associated Athanogene 1)
is a 31-kDa protein with an apparent molecular mass of 46 kDa that can bind to
Bcl-2 and enhance its anti-apoptotic effect, resulting in increased protection
from cell death induced by apoptotic stimuli. Bag-1 shares no significant
homology with Bcl-2 or other Bcl-2 family proteins. The C-terminal region of
Bag-1 binds the PDGF and HGF Receptor, and enhances growth factor-mediated
protection from apoptosis. This represents a link between growth factor
receptors and anti-apoptotic mechanisms.
Long Name
Bcl-2-associated Anthanogene-1
Alternate Names
Bag1
Gene Symbol
BAG1
Additional Bag-1 Products
Product Documents for Mouse Bag-1 Antibody
Product Specific Notices for Mouse Bag-1 Antibody
For research use only
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