Mouse BCMA/TNFRSF17 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF593
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Blockade of Receptor-ligand Interaction, Western Blot
Cited:
Flow Cytometry, Functional Assay, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BCMA/TNFRSF17
Met1-Thr49
Accession # O88472
Met1-Thr49
Accession # O88472
Specificity
Detects BCMA/TNFRSF17 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human BCMA is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Mouse BCMA/TNFRSF17 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 750 ng/mL of Recombinant Human APRIL/TNFSF13 (Catalog # 884-AP) to immobilized Recombinant Mouse BCMA/TNFRSF17 Fc Chimera (Catalog # 593-BC) coated at 1 µg/mL (100 µL/well). At 25 μg/mL, this antibody will block >90% of the binding.
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse BCMA/TNFRSF17 Fc Chimera (Catalog # 593-BC)
Sample: Recombinant Mouse BCMA/TNFRSF17 Fc Chimera (Catalog # 593-BC)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BCMA/TNFRSF17
BCMA, B cell maturation, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Mouse BCMA is a 185 amino acid (aa) protein consisting of a 49 aa extracellular domain, a 23 aa transmembrane domain, and a 113 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.
References
- Madry, C. et al. (1998) Int. Immunol. 10:1693.
- Gras, M. et al. (1995) Int. Immunol. 7:1093.
- Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
- Marsters, S. et al. (2000) Curr. Biol. 10:785.
- Thompson, J. et al. (2000) J. Exp. Med. 192:129.
Long Name
B Cell Maturation Factor
Alternate Names
CD269, TNFRSF13A, TNFRSF17
Gene Symbol
TNFRSF17
UniProt
Additional BCMA/TNFRSF17 Products
Product Documents for Mouse BCMA/TNFRSF17 Antibody
Product Specific Notices for Mouse BCMA/TNFRSF17 Antibody
For research use only
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