Mouse BCMA/TNFRSF17 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB593F

R&D Systems, part of Bio-Techne
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FAB593F
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MAB593
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 161616

View all BCMA/TNFRSF17 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse BCMA
Met1-Thr49
Accession # O88472

Specificity

Detects mouse BCMA in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) BCMA or rhTACI.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse BCMA/TNFRSF17 Fluorescein-conjugated Antibody

Detection of BCMA/TNFRSF17 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of BCMA/TNFRSF17 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse BCMA/TNFRSF17 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB593F) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining . View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse BCMA/TNFRSF17 Fluorescein-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Reviewed Applications

Read 3 reviews rated 4.7 using FAB593F in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: BCMA/TNFRSF17

BCMA (B Cell Maturation Antigen), also known as TNFRSF17, is a 22-26 kDa member of the TNFR superfamily. It is a type III transmembrane (TM) protein one that is synthesized as a type I TM protein that has a restricted expression pattern. Among the cells recognized to express BCMA are bone marrow plasma cells (PCs), adipocytes and stratum basale keratinocytes. At least some human BCMA is now known to be N-glycosylated, a modification that affects both its affinity for ligand, and its half-life on the cell surface. Mouse, by contrast, has no potential N-linked site, and any glycosylation would have to be either O-linked, or N-linked through the generation of an alternative splice form. In any case, and although the predicted MW of mouse BCMA is 20 kDa, it appears to run anomalously at 22-26 kDa in SDS-PAGE. Mouse BCMA is 185 amino acids (aa) in length. It contains a long, 115 aa cytoplasmic region and a short 49 aa extracellular domain characterized by two cysteine-rich segments. Ligands for BCMA are trimers, and include APRIL and BAFF homotrimers, plus APRIL:BAFF heterotrimers. In human, BCMA is known to be proteolytically cleaved and released, acting as an antagonist to APRIL AND BAFF signaling; in mouse, it is unclear if cleavage occurs. BCMA activation on PCs promotes PC survival, while on adipocytes, BCMA signaling downregulates adiponectin and leptin production, and on keratinocytes, BCMA ligation induces IL-6 and GM-CSF secretion. Over aa 1-49, mouse BCMA shares 65% and 90% aa sequence identity with human and rat BCMA, respectively.

Long Name

B Cell Maturation Factor

Alternate Names

CD269, TNFRSF13A, TNFRSF17

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Gene Symbol

TNFRSF17

UniProt

O88472

Additional BCMA/TNFRSF17 Products

Product Documents for Mouse BCMA/TNFRSF17 Fluorescein-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse BCMA/TNFRSF17 Fluorescein-conjugated Antibody

For research use only

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