Mouse beta2-Microglobulin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8325
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 893803
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse beta2‑Microglobulin
Met1-Met119
Accession # P01887
Met1-Met119
Accession # P01887
Specificity
Detects mouse beta2‑Microglobulin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse beta2-Microglobulin Antibody
Detection of Mouse beta2‑Microglobulin by Western Blot.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse beta2-Microglobulin Monoclonal Antibody (Catalog # MAB8325) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for beta2-Microglobulin at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse beta2-Microglobulin Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and Hepa 1‑6 mouse hepatoma cell line
Sample: RAW 264.7 mouse monocyte/macrophage cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and Hepa 1‑6 mouse hepatoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta 2-Microglobulin
Alternate Names
B2M, beta 2Microglobulin
Gene Symbol
B2M
UniProt
Additional beta 2-Microglobulin Products
Product Documents for Mouse beta2-Microglobulin Antibody
Product Specific Notices for Mouse beta2-Microglobulin Antibody
For research use only
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