Mouse BLAME/SLAMF8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4156
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BLAME/SLAMF8
Val21-Asp231
Accession # Q9D3G2
Val21-Asp231
Accession # Q9D3G2
Specificity
Detects mouse BLAME/SLAMF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human BLAME is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse BLAME/SLAMF8 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse BLAME/SLAMF8
Sample: Recombinant Mouse BLAME/SLAMF8
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BLAME/SLAMF8
BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of the Ig superfamily cell receptors. The 258 amino acid (aa) mature mouse BLAME contains a 211 aa extracellular domain (ECD) with an Ig V- and a C2-like domain, and a 26 aa cytoplasmic tail that lacks signaling motifs. BLAME is expressed by activated monocytes and dendritic cells. Although the function of BLAME is not known, it is likely involved in homotypic binding or heterotypic interactions with other CD2 subset members. Over the ECD region used as immunogen, mouse BLAME shares 95% and 78% aa identity with rat and human BLAME, respectively.
Long Name
SLAM Family Member 8
Alternate Names
CD353, SBBI42, SLAMF8
Gene Symbol
SLAMF8
UniProt
Additional BLAME/SLAMF8 Products
Product Documents for Mouse BLAME/SLAMF8 Antibody
Product Specific Notices for Mouse BLAME/SLAMF8 Antibody
For research use only
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