Mouse BMP-5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6176
Key Product Details
Species Reactivity
Mouse
Applications
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 781321
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse BMP-5
Ala315-His452
Accession # P49003
Ala315-His452
Accession # P49003
Specificity
Detects mouse BMP-5 in ELISA.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse BMP-5 Antibody
Alkaline Phosphatase Production Induced by BMP‑5 and Neutrali-zation by Mouse BMP‑5 Antibody.
Recombinant Mouse BMP-5 (6176-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse BMP-5 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse BMP-5 Mono-clonal Antibody (Catalog # MAB6176). The ND50 is typically 5-25 µg/mL.Applications for Mouse BMP-5 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize BMP‑5-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Asahina, I. et al. (1996) Exp. Cell Res. 222:38.The Neutralization Dose (ND50) is typically 5-25 μg/mL in the presence of 2 μg/mL Recombinant Mouse BMP‑5.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-5
References
- Chen, D. et al. (2004) Growth Factors 22:233.
- Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
- King, J.A. et al. (1994) Dev. Biol. 166:112.
- Mailhot, G. et al. (2008) J. Cell. Physiol. 214:56.
- Zuzarte-Luis, V. et al. (2004) Dev. Biol. 272:39.
- Ho, A.M. et al. (2008) BMC Dev. Biol. 8:35.
- Pierre, A. et al. (2005) Biol. Reprod. 73:1102.
- Beck, H.N. et al. (2001) BMC Neurosci. 2:12.
- Brederlau, A. et al. (2002) Mol. Cell. Neurosci. 21:367.
- Jin, Y. et al. (2001) Oral Oncol. 37:225.
- Ro, T.B. et al. (2004) Oncogene 23:3024.
Long Name
Bone Morphogenetic Protein 5
Alternate Names
BMP5
Gene Symbol
BMP5
UniProt
Additional BMP-5 Products
Product Documents for Mouse BMP-5 Antibody
Product Specific Notices for Mouse BMP-5 Antibody
For research use only
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