Mouse BTN2A2/Butyrophilin 2A2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4917

R&D Systems, part of Bio-Techne
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AF4917
AF4917-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all BTN2A2/Butyrophilin 2A2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse BTN2A2/Butyrophilin 2A2
Gln29-Ser219
Accession # A4QPC6.1

Specificity

Detects mouse BTN2A2/Butyrophilin 2A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than10% cross-reactivity with recombinant mouse (rm) BTN and rmBTNL2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse BTN2A2/Butyrophilin 2A2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse BTN2A2/Butyrophilin 2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTN2A2/Butyrophilin 2A2

Mouse Butyrophilin 2 (BTN2; also Btn2a2) is a 65‑70 kDa member of the BTN/MOG family of proteins. It is expressed on colonic epithelium and endothelial cells, and marginally on monocytes. The counterreceptor for BTN2 is DC-SIGN and binding is predicated on the presence of BTN2 mannose residues. Mature mouse BTN2 is presumably 487 amino acids (aa) in length. It is a type I transmembrane glycoprotein that potentially contains a 206 aa extracellular domain (ECD) (aa 29‑234). The ECD contains one V-type (aa 52‑129) and one C2-type (aa 152‑234) Ig-like domain. There is one alternative splice variant. It shows a deletion of aa 372‑400 plus a 17 aa substitution for aa 422‑451. Over aa 30‑248, mouse BTN2 shares 70% and 89% aa identity with human and rat BTN2, respectively.

Long Name

Butyrophilin Subfamily 2 Member A2

Alternate Names

BTF2, BTN2, Butyrophilin 2

Entrez Gene IDs

10385 (Human); 238555 (Mouse); 306957 (Rat)

Gene Symbol

BTN2A2

UniProt

A4QPC6.1

Additional BTN2A2/Butyrophilin 2A2 Products

Product Documents for Mouse BTN2A2/Butyrophilin 2A2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse BTN2A2/Butyrophilin 2A2 Antibody

For research use only

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