M1 mouse myeloid leukemia cell line was stained with Mouse C1q R1/CD93 Monoclonal Antibody (Catalog # MAB1696, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113).

Applications for Mouse C1qR1/CD93 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: M1 mouse myeloid leukemia cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed M1 mouse myeloid leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C1qR1/CD93

Complement component 1, q subcomponent receptor (C1q R1), also known as C1q Rp, Collectin receptor and AA4 antigen in rodents, mediates enhanced phagocytosis by monocytes and macrophages upon interaction with soluble defense collagens including C1q, MBL (mannose-binding lectin) and SP-A (pulmonary surfactant protein A) (1, 2). It is a type I transmembrane glycoprotein expressed on endothelial cells, hematopoietic progenitor cells, platelets and cells of myeloid origin. C1q R1 has also been identified as a stem cell marker (3, 4).

References

Kim, T.S. et al. (2000) Mol. Immunol. 37:377.
Dean, Y.D. et al. (2001) Eur. J. Immunol. 31:1370.
Petrenko, O. et al. (1999) Immunity 10:691.
Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:1044.

Long Name

Complement Component C1q Receptor

Alternate Names

AA4 Antigen, C1q R1, C1q Rp, C1qRp, CD93, Collectin Receptor, Ly68

Entrez Gene IDs

22918 (Human); 17064 (Mouse); 84398 (Rat)

Gene Symbol

CD93

UniProt

O89103

Additional C1qR1/CD93 Products

Product Documents for Mouse C1qR1/CD93 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse C1qR1/CD93 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse C1qR1/CD93 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1696

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all C1qR1/CD93 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse C1qR1/CD93 Antibody

Detection of C1q R1/CD93 in M1 Mouse Cell Line by Flow Cytometry.