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Key Product Details

Species Reactivity

Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 468705

Product Specifications

Immunogen

Chinese hamster ovary cell line transfected with mouse C5L2
Met1-Val344
Accession # Q8BW93

Specificity

This antibody stains mouse C5L2 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse C5L2/GPR77 Alexa Fluor® 488-conjugated Antibody

Detection of C5L2/GPR77 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of C5L2/GPR77 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse C5L2/GPR77 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC4729G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Mouse C5L2/GPR77 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: C5L2/GPR77

C5L2, also known as GPR77, is a 45 kDa 7TM receptor that binds the complement proteins C3a, C4a, and C5a as well as the acylation stimulating peptide (ASP). C5L2 is expressed on various hematopoietic cells where it promotes complement-mediated inflammation. ASP binding to C5L2 on adipocytes triggers triglyceride synthesis and glucose uptake and inhibits lipolysis. Human C5L2 shares 60% amino acid sequence identity with mouse and rat C5L2.

Long Name

C5a Anaphylatoxin Chemotactic Receptor C5L2

Alternate Names

GPR77

Entrez Gene IDs

27202 (Human); 319430 (Mouse); 445269 (Rat)

Gene Symbol

C5AR2

UniProt

Additional C5L2/GPR77 Products

Product Documents for Mouse C5L2/GPR77 Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse C5L2/GPR77 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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