Mouse Cadherin-4/R-Cadherin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6677
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Xenograft
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cadherin-4/R-Cadherin
His21-Ala731
Accession # P39038
His21-Ala731
Accession # P39038
Specificity
Detects mouse Cadherin-4/R-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Cadherin-4/R-Cadherin and recombinant mouse (rm) Cadherin-2 is observed and less than 1% cross-reactivity with rmE‑Cadherin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Cadherin-4/R-Cadherin Antibody
Detection of Mouse Cadherin‑4/R‑Cadherin by Western Blot.
Western blot shows lysates of mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Cadherin-4/R-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6677) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cadherin-4/R-Cadherin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Cadherin‑4/R‑Cadherin in Mouse Brain.
Cadherin-4/R-Cadherin was detected in immersion fixed frozen sections of embryonic mouse brain using Sheep Anti-Mouse Cadherin-4/R-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6677) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Cadherin-4/R-Cadherin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse brain
Sample: Immersion fixed frozen sections of embryonic mouse brain
Western Blot
1 µg/mL
Sample: Mouse brain (cortex) tissue
Sample: Mouse brain (cortex) tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cadherin-4/R-Cadherin
Cadherin-4 (also Retinal/R-Cadherin) is a 120 kDa (100 kDa predicted) member of the classical cadherin family of molecules. It is expressed on mammary epithelium, olfactory bulb neurons, cerebellar granule cells, vascular smooth muscle, astrocytes and pancreatic beta-cells. Cadherin-4 forms both homodimers and heterodimers with N-cadherin. These complexes will oligomerize, providing sources for adhesion and migration. Cadherin-4 also binds to KLRG1 expressed on NK, T and Treg lymphocytes. Mouse cadherin-4 proprecursor is 893 amino acids (aa) in length. It contains a 146 aa propeptide (aa 21-166) coupled to a 746 amino acid (aa) type I transmembrane mature protein (aa 167-913). The mature region possesses a 565 aa extracellular domain (aa 167-731) plus a 160 aa cytoplasmic region (aa 753-913). Consistent with classical cadherins, cadherin-4 contains five cadherin repeats in the extracellular domain (aa 167-721). There is one potential splice form that shows a 44 aa substitution for aa 291-913. Over aa 21-731, mouse cadherin-4 shares 98% and 93% aa identity with rat and human cadherin-4, respectively.
Alternate Names
CAD4, Cadherin4, CDH4, R-Cadherin
Gene Symbol
CDH4
UniProt
Additional Cadherin-4/R-Cadherin Products
Product Documents for Mouse Cadherin-4/R-Cadherin Antibody
Product Specific Notices for Mouse Cadherin-4/R-Cadherin Antibody
For research use only
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