Mouse Carbonic Anhydrase IV/CA4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2414
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat, Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, ISH-IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IV/CA4
Glu18-Ser277
Accession # Q64444
Glu18-Ser277
Accession # Q64444
Specificity
Detects mouse Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human CA4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Carbonic Anhydrase IV/CA4 Antibody
Detection of Mouse Carbonic Anhydrase IV/CA4 by Western Blot.
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Carbonic Anhydrase IV/CA4 in Mouse Kidney.
Carbonic Anhydrase IV/CA4 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Carbonic Anhydrase IV/CA4 by Simple WesternTM.
Simple Western lane view shows lysates of mouse lung tissue and mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 51-52 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse Carbonic Anhydrase IV/CA4 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney
Sample: Perfusion fixed frozen sections of mouse kidney
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Carbonic Anhydrase IV (Catalog # 2414-CA), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Carbonic Anhydrase IV (Catalog # 2414-CA), see our available Western blot detection antibodies
Simple Western
12.5 µg/mL
Sample: Mouse lung tissue and mouse kidney tissue
Sample: Mouse lung tissue and mouse kidney tissue
Western Blot
0.25 µg/mL
Sample: Mouse kidney tissue
Sample: Mouse kidney tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase IV/CA4
Carbonic anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. The deduced amino acid sequence of mouse CA4 consists of a signal peptide (residues 1‑17), an ectodomain (residues 18‑277) and a pro peptide (residues 278‑305), which is removed in the mature form (2). The native enzyme is attached to the membrane by a GPI‑anchor. Recombinant mouse CA4 corresponds to the ectodomain and exhibits the activity as described in Activity Assay Protocol.
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Tamai, S. et al. (1996) Biochem. Genet. 34:31.
Alternate Names
CA4, RP17
Gene Symbol
CA4
UniProt
Additional Carbonic Anhydrase IV/CA4 Products
Product Documents for Mouse Carbonic Anhydrase IV/CA4 Antibody
Product Specific Notices for Mouse Carbonic Anhydrase IV/CA4 Antibody
For research use only
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