Mouse Cathepsin D Antibody

Detection of Human Cathepsin D by Western Blot

Chronic treatment with BACE1 inhibitors causes accumulation of CatD.(a) Representative western blot of brain homogenates prepared from mice dosed daily for 30 days with PF-9283 (80 mg kg−1) showed increased levels of CatD heavy and light chains, but not the CatD single chain, compared with a vehicle control. Data are presented as mean (normalized to actin)±s.e.m.; n=5 (PF-9283) and n=6 (veh) per group. (b) ELISA of live ARPE-19 cells incubated with BACE1 inhibitors for 7 days showed accumulation of CatD; the extent of accumulation was consistent with cellular CatD IC50 values (Supplementary Table 4). Data are presented as mean±s.e.m. for three independent experiments. (c) Proteomic analysis of RPE tissue prepared from rats dosed daily for 14 days with 80 mg kg−1 PF-9283. Peptide spectral matches (PSM) of 11 of 391 identified proteins were changed more than 1.5-fold (P<0.01, red symbols, see also Supplementary Table 5) compared with vehicle controls including a 1.7-fold increase in CatD (***P=0.0002). Average PSM were calculated from six LC-MS runs consisting of two technical replicates of n=3 RPE tissues per group. For a–c, significant differences were determined using a two-tailed t-test; *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27727204), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human Cathepsin D by Western Blot

Chronic treatment with BACE1 inhibitors causes accumulation of CatD.(a) Representative western blot of brain homogenates prepared from mice dosed daily for 30 days with PF-9283 (80 mg kg−1) showed increased levels of CatD heavy and light chains, but not the CatD single chain, compared with a vehicle control. Data are presented as mean (normalized to actin)±s.e.m.; n=5 (PF-9283) and n=6 (veh) per group. (b) ELISA of live ARPE-19 cells incubated with BACE1 inhibitors for 7 days showed accumulation of CatD; the extent of accumulation was consistent with cellular CatD IC50 values (Supplementary Table 4). Data are presented as mean±s.e.m. for three independent experiments. (c) Proteomic analysis of RPE tissue prepared from rats dosed daily for 14 days with 80 mg kg−1 PF-9283. Peptide spectral matches (PSM) of 11 of 391 identified proteins were changed more than 1.5-fold (P<0.01, red symbols, see also Supplementary Table 5) compared with vehicle controls including a 1.7-fold increase in CatD (***P=0.0002). Average PSM were calculated from six LC-MS runs consisting of two technical replicates of n=3 RPE tissues per group. For a–c, significant differences were determined using a two-tailed t-test; *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27727204), licensed under a CC-BY license. Not internally tested by R&D Systems.