Mouse CCL11/Eotaxin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-420-NA
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Neutralization, Western Blot
Cited:
ELISA Development, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Luminex Development, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL11/Eotaxin
His24-Pro97
Accession # P48298
His24-Pro97
Accession # P48298
Specificity
Detects mouse CCL11/Eotaxin in ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human CCL11/Eotaxin is observed, and less than 5% cross-reactivity with recombinant mouse JE is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL11/Eotaxin Antibody
Chemotaxis Induced by CCL11/Eotaxin and Neutral-ization by Mouse CCL11/ Eotaxin Antibody.
Recombinant Mouse CCL11/ Eotaxin (Catalog # 420-ME) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL11/ Eotaxin (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA). The ND50 is typically 0.1-0.5 µg/mL.
CCL11/Eotaxin in Mouse Thymus.
CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
CCL11/Eotaxin in Mouse Colon.
CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse CCL11/Eotaxin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus and mouse colon
Sample: Perfusion fixed frozen sections of mouse thymus and mouse colon
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME)
Sample: Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME)
Neutralization
Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 10 ng/mL Recombinant Mouse CCL11/Eotaxin.
Mouse CCL11/Eotaxin Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.7 using AF-420-NA in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL11/Eotaxin
CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Mouse CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high amino acid sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor.
References
- Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
- Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
- Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
- Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
Alternate Names
Eotaxin
Gene Symbol
CCL11
UniProt
Additional CCL11/Eotaxin Products
Product Documents for Mouse CCL11/Eotaxin Antibody
Product Specific Notices for Mouse CCL11/Eotaxin Antibody
For research use only
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