Mouse CCL17/TARC Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB529
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Cell Selection, Direct ELISA, ELISA Development, Luminex Development, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 110904
Product Specifications
Immunogen
E. coli-derived Recombinant Mouse CCL17/TARC
Ala24-Pro93
Accession # Q9WUZ6
Ala24-Pro93
Accession # Q9WUZ6
Specificity
Detects mouse CCL17/TARC in ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL17/TARC Antibody
Chemotaxis Induced by CCL17/TARC and Neutralization by Mouse CCL17/TARC Antibody.
Recombinant Mouse CCL17/TARC (Catalog # 529-TR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL17/TARC (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL17/TARC Monoclonal Antibody (Catalog # MAB529). The ND50 is typically 0.75-3.0 µg/mL.
Detection of Mouse CCL17/TARC by Block/Neutralize
CCL17-mediated chemotaxis is inhibited in a dose-dependent manner by B202 and B225.EL4 cells were incubated with mouse CCL17 at 2/mL in the presence and absence of (A) B202, (B) B225 (C) CNTO 5516 which is an irrelevant rat:mouse chimeric antibody having a mouse IgG1 Fc or (D) MAB529 (rat antibody) which is a commercial antibody that neutralizes CCL17. Assay was repeated twice with five replicates per sample each time. Data are expressed as chemotactic index (CI = Average RLU from stimulated wells/average RLU from medium control; medium control = CI of 1). Antibody dose used for each sample is indicated along the x axis. Composite CI results from both experiments are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24339934), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse CCL17/TARC Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL17/TARC.
Mouse CCL17/TARC Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB529 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL17/TARC
Human thymus and activation-regulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse CCL17 was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse CCL17 cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse CCL17 is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant CCL17 has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, CCL17 was identified to be a specific functional ligand for CCR4 and CCR8, receptors that are selectively expressed on T cells.
References
- Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
- Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
- Nomiyama, H. et al. (1997) Genomics 40:211.
- Lieberam, I. et al. (1999) Eur. J. Immunol. 29:2684.
Alternate Names
ABCD-2, SCYA17, TARC
Gene Symbol
CCL17
UniProt
Additional CCL17/TARC Products
Product Documents for Mouse CCL17/TARC Antibody
Product Specific Notices for Mouse CCL17/TARC Antibody
For research use only
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