Mouse CCL24/Eotaxin-2/MPIF-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB528
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Cited:
ELISA Development, ELISA Development (Capture), Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 106521
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2
Ile24-Val119
Accession # Q9JKC0
Ile24-Val119
Accession # Q9JKC0
Specificity
Detects mouse CCL24/Eotaxin‑2/MPIF‑2 in Western blots. In Western blots, no cross-reactivity with
recombinant human
CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,
28, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22,
25, 28, or recombinant rat CCL20 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody
Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutral-ization by Mouse CCL24/ Eotaxin‑2 Antibody.
Recombinant Mouse CCL24/ Eotaxin-2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin-2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL24/Eotaxin-2 Monoclonal Antibody (Catalog # MAB528). The ND50 is typically 0.06-0.3 µg/mL.Applications for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (Catalog # 528-MP)
Sample: Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (Catalog # 528-MP)
Neutralization
Measured by its ability to neutralize CCL24/Eotaxin-2/MPIF-2-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.06-0.3 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL24/Eotaxin-2/MPIF-2.
Mouse CCL24/Eotaxin-2/MPIF-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL24/Eotaxin-2/MPIF-2
Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to CCL11/Eotaxin and CCL26/Eotaxin-3. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin.
Alternate Names
Eotaxin-2, MPIF-2, MPIF2
Gene Symbol
CCL24
UniProt
Additional CCL24/Eotaxin-2/MPIF-2 Products
Product Documents for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody
Product Specific Notices for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody
For research use only
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