Mouse CCL24/Eotaxin-2/MPIF-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF528
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry-Paraffin, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2
Ile24-Val119
Accession # Q9JKC0
Ile24-Val119
Accession # Q9JKC0
Specificity
Detects CCL24/Eotaxin‑2/MPIF‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Eotaxin-2 is observed, and less than 1% cross-reactivity with recombinant mouse Eotaxin is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody
Chemotaxis Induced by CCL24/Eotaxin-2 and Neutralization by Mouse CCL24/Eotaxin-2 Antibody.
Recombinant Mouse CCL24/Eotaxin-2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin-2 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL24/ Eotaxin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF528). The ND50 is typically 0.5-2.5 µg/mL.Applications for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (Catalog # 528-MP)
Sample: Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (Catalog # 528-MP)
Neutralization
Measured by its ability to neutralize CCL24/Eotaxin-2/MPIF-2-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.02 µg/mL Recombinant Mouse CCL24/Eotaxin-2/MPIF-2.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL24/Eotaxin-2/MPIF-2
Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to eotaxin/CCL11 and Eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin.
Alternate Names
Eotaxin-2, MPIF-2, MPIF2
Gene Symbol
CCL24
UniProt
Additional CCL24/Eotaxin-2/MPIF-2 Products
Product Documents for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody
Product Specific Notices for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody
For research use only
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