Mouse CCL3/MIP-1 alpha APC-conjugated Antibody

Detection of CCL3/MIP‑1 alpha in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line treated with LPS was stained with Rat Anti-Mouse CCL3/MIP-1a APC-conjugated Monoclonal Antibody (Catalog # IC450A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Mouse CCL3/MIP-1 alpha by Flow Cytometry

Maternally derived H-2Dd also impedes fetal growth and vascular remodelling.(a) Density plots showing the proportion of IFN-gamma + or MIP-1 alpha+ uNK among cells positive or negative for Ly49A. (b–e) Fold-change in IFN-gamma - and MIP-1 alpha-producing cells among uNK expressing Ly49A compared with cells that do not. (b) Proportion of IFN-gamma + cells among cells expressing Ly49A compared with Ly49A−uNK (irrespective of expression of other Ly49). (c) Proportion of IFN-gamma + cells among cells expressing Ly49A compared with Ly49A−uNK (after exclusion of Ly49C/I+ cells). (d) Proportion of MIP-1 alpha+ cells among cells expressing Ly49A compared with Ly49A−uNK (irrespective of expression of other Ly49). (e) Proportion of MIP-1 alpha+ cells among cells expressing Ly49A compared with Ly49A−uNK (after exclusion of Ly49C/I+ cells). Pooled data from four experiments, n=6 mice. P-values comparing uNK subset expressing a given Ly49 with those that do not within each mouse using paired Student’s t-tests. Means±s.e.m. (f) Presence of smooth muscle actin around spiral arteries. Scale bar=100 μm. (g–j) Fetal growth is reduced in D8 females mated with either B6 (g) or D8 (i) males. Weights expressed as fraction of fetal weight in B6 × B6 controls. P-values for the effect of parental cross using a mixed model approach (taking into account inter-litter variability). Proportion of fetuses in or below the bottom 5% rank of controls (that is, ≤5th percentile) for D8 females mated with either B6 (h) or D8 (j) males. P-value from a χ2 test comparing the observed fraction with the expected value of 5%. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms4359), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse CCL3/MIP-1 alpha by Flow Cytometry

Paternal MHC does not educate uNK cells.(a) Density plots showing the proportion of IFN-gamma producing uNK (gd9.5+10.5) in response to NK1.1 cross-linking among cells positive or negative for the indicated Ly49 (irrespective of expression of other Ly49). (b–d) Fold change in IFN-gamma producing cells among uNK expressing the indicated Ly49 compared with cells that do not. Ly49C/I (b), Ly49A (c) and Ly49A after exclusion of Ly49C/I+ cells (d). (e) Density plots showing MIP-1 alpha producing uNK cells (gd9.5+10.5) in response to NK1.1 cross-linking among cells positive or negative for the indicated Ly49 (irrespective of expression of other Ly49). (f–h) Fold-change in MIP-1 alpha producing cells among uNK expressing the indicated Ly49 compared with cells that do not. Ly49C/I (f), Ly49A (g) and Ly49A after exclusion of Ly49C/I+ cells (h). Pooled data from two experiments, n=6–11 mice per group. P-values comparing uNK subset expressing a given Ly49 with those that do not within each mouse using paired Student’s t-tests. Means±s.e.m. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms4359), licensed under a CC-BY license. Not internally tested by R&D Systems.