Mouse CCR5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6138
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 645807
Product Specifications
Immunogen
E.coli-derived recombinant mouse CCR5
Met1-Ala32
Accession # P51682
Met1-Ala32
Accession # P51682
Specificity
Detects CCR5 in direct ELISAs and Western blots. No cross-reactivity with recombinant mouse CCR1, 4, or 8 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse CCR5 Antibody
Detection of Mouse CCR5 by Western Blot.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line treated (+) with 100 µg/mL LPS for 4 hours. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse CCR5 Monoclonal Antibody (Catalog # MAB6138) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCR5 at approximately 50 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse CCR5 Antibody
Application
Recommended Usage
Western Blot
RAW 264.7 mouse monocyte/macrophage cell line treated with LPS under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR5
CCR5 (CC chemokine receptor 5; also CD195 and MIP-1a receptor) is a 41 - 44 kDa member of the G-protein coupled receptor #1 family of proteins. It is expressed on Treg cells, NK cells, neurons, macrophages and Th1 cells. CCR5 mediates cell adhesion and migration induced by several chemokines including CCL3/MIP-1 alpha, CCL4/MIP-1 beta, CCL5/RANTES, and CCL8/MCP-2. It also functions as a coreceptor for macrophage-tropic HIV-1 infection. CCR5 contains an O-glycosylated and sulfated extracellular N-terminus (aa 1 - 32), and a phosphorylated and palmitoylated intracellular C-terminus (aa 304 - 354). CCR5 will form homodimers, heterodimers with CCR2, and heterooligomers with CCR2 and CXCR4. Within aa 1 - 32, mouse CCR5 shares 72% and 91% amino acid sequence identity with human and rat CCR5, respectively.
Alternate Names
CCR5, CD195
Gene Symbol
CCR5
UniProt
Additional CCR5 Products
Product Documents for Mouse CCR5 Antibody
Product Specific Notices for Mouse CCR5 Antibody
For research use only
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