Mouse CCR7 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3477A

R&D Systems, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 4B12

View all CCR7 Antibodies »

Product Specifications

Immunogen

RBC-2H3 cells expressing mouse CCR7
Accession # NP_031745

Specificity

Detects mouse CCR7.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CCR7 APC-conjugated Antibody

Detection of CCR7 antibody in Mouse CD4+Splenocytes antibody by Flow Cytometry.

Detection of CCR7 in Mouse CD4+Splenocytes by Flow Cytometry.

Mouse CD4+splenocytes were stained with Rat Anti-Mouse CCR7 APC-conjugated Monoclonal Antibody (Catalog # FAB3477A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse CCR7 by Flow Cytometry

Detection of Mouse CCR7 by Flow Cytometry

B6 and CD69−/− CD4 T cells differ in the expression of naïve and memory surface cell markers.Cells were isolated from the spleen (spl), mesenteric lymph nodes (MLN), small intestinal lamina propria (siLP), colonic lamina propria (cLP) and blood (bld) of non-treated B6 and CD69−/− mice and analyzed by flow cytometry. The surface expression of CD44 (A), CD103 (B), CD62L (C), CCR-7 (D), CCR-9 (E) and alpha4 beta7 integrin (F) by CD4 T cells were analysed. Graphs represent mean (± SEM) of CD4 T cell fraction expressing the indicated molecule for four mice per each strain per tissue. Black bars represent data for B6 and white bars for CD69−/− cells. *p≤0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23776480), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CCR7 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse CD4+ splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR7

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Mouse CCR7 shares 87% amino acid sequence identity with human CCR7.

Alternate Names

BLR2, CC-CKR-7, CCR7, CD197, CDw197, CMKBR7, EBI1

Entrez Gene IDs

1236 (Human); 12775 (Mouse); 287673 (Rat)

Gene Symbol

CCR7

UniProt

NP_031745

Additional CCR7 Products

Product Documents for Mouse CCR7 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCR7 APC-conjugated Antibody

For research use only

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