HEK293 human embryonic kidney cell line transfected with mouse CCR8 and eGFP (top panel) or irrelevant transfectant (bottom panel) were stained with Rabbit Anti-Mouse CCR8 PE-conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # FAB8324P). Quadrant markers were set based on control antibody staining (Catalog # IC105P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CCR8 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse CCR8 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using FAB8324P in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR8

CCR8 (C-C chemokine Receptor 8; also known as CD198) is a 41-43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8⁺ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Mouse CCR8 is a 7-transmembrane protein that is 353 amino acids (aa) in length. It contains a 33 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. In mouse, CCR8 is N- and possibly O-glycosylated, and known to be sulfated on Tyr14 and 15. The unusual nature of these posttranslational modifications may lead to anomalous migration in SDS-PAGE. Over aa sequences 1-33 and 92-105 collectively, mouse CCR8 shares 64% and 85% aa identity with human and rat CCR8, respectively.

Alternate Names

CCR8, CD198, CDw198, ChemR1, CKRL1, GPRCL1, TER-1

Entrez Gene IDs

1237 (Human); 12776 (Mouse)

Gene Symbol

CCR8

UniProt

NP_031746

Additional CCR8 Products

Product Documents for Mouse CCR8 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse CCR8 PE-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Mouse CCR8 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8324P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CCR8 PE-conjugated Antibody

Detection of CCR8 in HEK293 Human Cell Line Transfected with Mouse CCR8 and eGFP by Flow Cytometry.