Mouse CD11c Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6950
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 435421
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD11c
Phe20-Pro1116
Accession # Q9QXH4
Phe20-Pro1116
Accession # Q9QXH4
Specificity
Detects mouse CD11c in direct ELISAs and Western blots. In direct ELISAs, 50%‑100% cross‑reactivity with recombinant human (rh) Integrin alphaX beta2 and recombinant mouse (rm) Integrin alphaL is observed, 10%‑25% cross‑reactivity with rhIntegrin CD11c, rhIntegrin alphaD beta2, and rhIntegrin alphaL beta2 is observed and no cross-reactivity with rhIntegrin alphaM or rhIntegrin beta2 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse CD11c Antibody
Detection of Mouse CD11c by Western Blot.
Western blot shows lysates of mouse lymph node tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB6950) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD11c at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CD11c in Mouse Lung.
CD11c was detected in immersion fixed paraffin-embedded sections of mouse lung using Rat Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB6950) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to macrophages. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Mouse CD11c Antibody
Application
Recommended Usage
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse lung
Sample: Immersion fixed paraffin-embedded sections of mouse lung
Western Blot
2 µg/mL
Sample: Mouse lymph node tissue
Sample: Mouse lymph node tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11c
Alternate Names
CD11c, Integrin alpha X, ITGAX, p150,95 alpha
Gene Symbol
ITGAX
UniProt
Additional CD11c Products
Product Documents for Mouse CD11c Antibody
Product Specific Notices for Mouse CD11c Antibody
For research use only
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