Mouse CD14 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF982

R&D Systems, part of Bio-Techne
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BAF982
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AF982
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all CD14 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD14
Ala18-Pro345
Accession # Q4FJP7

Specificity

Detects mouse CD14 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human CD14 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse CD14 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD14 Fc Chimera (Catalog # 982-CD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The mouse CD14 cDNA encodes a 366 amino acid (aa) residue precursor protein with a 15 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Mouse CD14 has five potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of mouse CD14 is approximately 65% and 82% identical to the human and rat proteins, respectively. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

References

  1. Wright, S.D. et al. (1990) Science 249:1431.
  2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
  3. Beutler, B. (2000) Current Opinion in Immunology 12:20.
  4. Stelter, F. (2000) Chem. Immunol. 74:25.

Alternate Names

CD14

Entrez Gene IDs

929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)

Gene Symbol

CD14

UniProt

Q4FJP7

Additional CD14 Products

Product Documents for Mouse CD14 Biotinylated Antibody

Product Datasheets

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD14 Biotinylated Antibody

For research use only

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