HEK293 human embryonic kidney cell line transfected with (A) mouse CD200R1L or (B) irrelevant protein, and eGFP was stained with Rabbit Anti-Mouse CD200R1L Monoclonal Antibody (Catalog # MAB10198) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.

CD200R1L in Mouse Kidney.

CD200R1L was detected in immersion fixed paraffin-embedded sections of mouse kidney using Rabbit Anti-Mouse CD200R1L Monoclonal Antibody (Catalog # MAB10198) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse CD200R1L Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse CD200R1L and eGFP

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse kidney

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD200R1L

CD200 Receptor 2 (CD200R2), or CD200R1L, is a type I transmembrane receptor for the CD200 cell surface glycoprotein. Mature CD200R2 consists of an extracellular domain (ECD) containing an Ig-like V-type domain and an Ig-like C2-type domain, a transmembrane segment and a short cytoplasmic domain. The ECD of mouse CD200R2 shares a 76% and 63% amino acid (aa) identity with the rat and human ECD, respectively. CD200R2 is strongly expressed on resting mast cells but deceased upon activation via Fc epsilonR1. It is also observed on monocytes, bone marrow-derived dendritic cells and TH2 cells (1, 2, 4). It has been demonstrated CD200R2 can interact with CD200 (3) and signal through adaptor protein, DAP12, via its lysine residue in the transmembrane region (2). In addition, it has been reported CD200 and CD200R2 interaction alters dendritic cell differentiation and enhances induction of CD4⁺CD25⁺Foxp3⁺ regulatory T cells in mouse transplant model (5).

References

Wright, G. et al. (2003) J. Immunol. 171:3034.
Voehringer, D. (2004) J. Biol. Chem. 279(52):54117.
Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
Minas, K. (2006) Crit Rev Immunol 26(3):213.
Gorczynski, R. et al. (2008) J. Immunol. 180(9):5946.

Long Name

CD200 Receptor 1 Like

Alternate Names

CD200R2, CD200RLa

Entrez Gene IDs

344807 (Human); 271375 (Mouse); 501779 (Rat)

Gene Symbol

CD200R1L

UniProt

Q6XJV6.1

Additional CD200R1L Products

Product Documents for Mouse CD200R1L Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse CD200R1L Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CD200R1L Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10198

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD200R1L Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD200R1L Antibody

Detection of CD200R1L in HEK293 Human Cell Line Transfected with Mouse CD200R1L and eGFP by Flow Cytometry