Mouse CD27/TNFRSF7 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5741P

R&D Systems, part of Bio-Techne
Discontinued Product
FAB5741P has been discontinued. View all CD27/TNFRSF7 products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 137915

View all CD27/TNFRSF7 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD27/TNFRSF7
Thr21-Arg182
Accession # P41272

Specificity

Detects mouse CD27 in direct ELISAs and Western blots. In Western blots; does not cross-react with recombinant human (rh) CD27, recombinant mouse (rm) CD40, rmGITR, rhDR6, rhTRAIL R4, rmHVEM, rmCD30, rmRANK, rmFas, rmTRAIL R2, rhTRADD, rm4-1BB, rmLT beta R, rmOPG, rmNGFR, rmDR3, rmBAFF R, rhTRAIL R3, rmTAJ, rmEDAR, rmOX-40, rmTWEAK R, rmTNF R1, rhRELT,  or rmTRAILR1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CD27/TNFRSF7 PE-conjugated Antibody

Detection of CD27/TNFRSF7 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD27/TNFRSF7 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD27/TNFRSF7 PE-conjugated Monoclonal Antibody (Catalog # FAB5741P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD27/TNFRSF7 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Reviewed Applications

Read 1 review rated 4 using FAB5741P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD27/TNFRSF7

CD27 is a 50-55 kDa lymphocyte-specific member of the Tumor Necrosis Factor Receptor Superfamily (TNFRSF) and is also known as TNFRSF7. Mouse CD27 cDNA encodes a 250 amino acid (aa) residue type I transmembrane protein with a 20 aa putative signal peptide, a 162 aa extracellular region containing three TNFR cysteine-rich repeats, a 21 aa transmembrane domain and a 47 aa cytoplasmic region. Over aa 21-182, mouse CD27 shares approximately 84% and 60% amino acid sequence identity with rat and human CD27, respectively. CD27 exists as a homodimer on the cell surface due to an extracellular disulfide bond in the membrane-proximal region. A soluble 32 kDa form of CD27 is also produced during the immune response and is found in various body fluids. CD27 is expressed on subsets of NK, T and B cells. This includes delta zeta T cells, CD25+ T regs, immature CD11blo NK cells, and resting plus effector CD8+ T cells. The expression of CD27 is upregulated upon T cell activation. Although CD27 appears to be a marker for human memory B cells, it is only expressed in a small population of mouse B cells in germinal centers and at sites of B cell stimulation, suggesting that mouse CD27 may be a marker for activated B cells. CD27 interacts with CD27 ligand (also named CD70 and TNFSF7), which is a member of the TNF ligand superfamily. Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. Ligation of CD27 on B cells has been shown to both inhibit the terminal differentiation of activated mouse B cells into plasma cells.

Alternate Names

CD27, TNFRSF7

Entrez Gene IDs

939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

Gene Symbol

CD27

UniProt

P41272

Additional CD27/TNFRSF7 Products

Product Documents for Mouse CD27/TNFRSF7 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse CD27/TNFRSF7 PE-conjugated Antibody

For research use only

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