Mouse CD302/CLEC13A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB64241

R&D Systems, part of Bio-Techne
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MAB64241
MAB64241-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Flow Cytometry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 662714

View all CD302/CLEC13A Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD302/CLEC13A
Met1-Cys165
Accession # Q9DCG2

Specificity

Detects mouse CD302/CLEC13A in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) CD302 is observed. No cross-reactivity with rhCLEC1, 2, 2A, 3B, 9A, 10A, 12B, 14A, recombinant mouse CLEC4B2, 9A, or 12A is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse CD302/CLEC13A Antibody

Detection of Mouse CD302/CLEC13A antibody by Western Blot.

Detection of Mouse CD302/CLEC13A by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 2 µg/mL of Mouse CD302/CLEC13A Monoclonal Antibody (Catalog # MAB64241) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD302/CLEC13A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1

Applications for Mouse CD302/CLEC13A Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD302/CLEC13A

CD302, also known as CLEC13A and DCL-1, is a 35 kDa type I transmembrane glycoprotein in the C-type lectin family. CD302 is expressed on monocytes, macrophages, dendritic cells, and granulocytes. It contains one carbohydrate binding domain in its extracellular region and can mediate antigen uptake. In human Hodgkin’s disease, a fusion protein containing portions of CD302 and the ECD of DEC-205 is expressed on Reed-Sternberg cells.  Within the ECD, mouse CD302 shares 82% and 92% aa sequence identity with human and rat CD302, respectively.

Alternate Names

BIMLEC, CD302, CLEC13A, DCL1

Entrez Gene IDs

9936 (Human); 66205 (Mouse); 295629 (Rat)

Gene Symbol

CD302

UniProt

Q9DCG2

Additional CD302/CLEC13A Products

Product Documents for Mouse CD302/CLEC13A Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD302/CLEC13A Antibody

For research use only

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