Mouse CD36/SR-B3 Antibody

Detection of Mouse CD36/SR-B3 by Western Blot

Liver GADD45 beta controls liver fatty acid handling by cytosolic FABP1 retentionA, BMale GADD45 beta+/+ (WT; n = 16) or GADD45 beta−/− (KO; n = 15) mice fasted for 24 h (fasted) with (AV‐G45b OE) or without (AV‐NC) liver‐restricted GADD45 beta over‐expression (n = 7–8/group). Liver mRNA expression of Gadd45b (A) as well as fatty acid metabolic genes (B) encompassing transport (Slc28a2, Slc27a5, Cd36), intracellular binding (Fabp1, Dbi) and metabolism (Acly, Dgat1, Atgl, Hsl).CRepresentative immunoblots of FATP2, CD36, FABP1 and GADD45 beta from liver whole tissue lysate (W) as well as fractionated organelles/intracellular structures including nuclei (N), mitochondria (MT), microsomes (MS) and cytoplasm (C), from GADD45 beta+/+ (WT) and GADD45 beta−/− (KO) mice.DQuantified band densities of FABP1 enrichment from fractions in C (n = 4/group).ELiver fraction enrichment of FABP1 from male GADD45 beta+/+ (WT) or GADD45 beta−/− (KO) mice fasted for 24 h with (AD‐G45b OE) or without (AD‐NC) liver‐restricted GADD45 beta over‐expression (n = 4/group). Insert shows a representative FABP1 immunoblot.FLiver fraction enrichment of FABP1 from obese/diabetic male db/db mice fasted for 24 h with (AD‐G45b OE) or without (AD‐NC) liver‐restricted GADD45 beta over‐expression (n = 4/group). Insert shows a representative FABP1 immunoblot.GFABP1 and GADD45B immunoblots from Flag immunoprecipitations (IP‐FLAG) or mock IP (IP‐HA) from liver input samples from mice with (AD‐G45b OE) or without (AD‐NC) liver‐restricted GADD45 beta over‐expression. Shown is a representative immunoblot from 3 separate experiments using 3 different input samples per condition.H–JLiver tissue long‐chain acyl‐CoA (LC‐acyl‐CoA) concentrations were determined in GADD45 beta+/+ (WT) or GADD45 beta−/− (KO) mice (H; n = 6/group) with (AD‐G45b OE) or without (AD‐NC) liver‐restricted GADD45 beta over‐expression (I; n = 5/group). Liver LC‐acyl‐CoA concentrations were determined in wild‐type (WT; C57Bl/6J) or obese/diabetic (db/db; BKS.Cg‐m+/+ Lepr DB/J) mice with (AD‐G45b OE) or without (AD‐NC) liver‐restricted GADD45 beta over‐expression (J; n = 4/group).Data information: Data are mean ± SEM. Effect of genotype, *P < 0.05, **P < 0.01, ***P < 0.001. Effect of viral manipulation: #P < 0.05, ##P < 0.01, ###P < 0.001. The statistical test used and respective P‐value outputs can be found in Appendix Table S1. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27137487), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse CD36/SR-B3 by Western Blot

HIIT improves lipid metabolism of skeletal muscle in T2DM mice. (A,D) Protein expressions of ACC, HMGCR, CPT-1 alpha, CD36, and internal control alpha-tubulin in skeletal muscle. (B,C,E,F) Quantification of proteins described in (A,D) with normalization to protein levels of alpha-tubulin. All data are presented as mean ± SEM. n = 4 per group; *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32922365), licensed under a CC-BY license. Not internally tested by R&D Systems.