Mouse CD4 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM554

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Cited:

Immunohistochemistry-Frozen

Label

Biotin

Antibody Source

Monoclonal Rat IgG2B Clone # GK1.5

View all CD4 Antibodies »

Product Specifications

Immunogen

Mouse CTL clone V4

Specificity

Recognizes mouse CD4 (1‑3).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CD4 Biotinylated Antibody

CD4 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD4 in Mouse Splenocytes.

CD4 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD4 Biotinylated Monoclonal Antibody (Catalog # BAM554) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI(blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse CD4 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Immunocytochemistry

8-25 µg/mL
Sample:  Immersion fixed mouse splenocytes

Immunohistochemistry

Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280., Zheng, B.S. et al. (1996) J. Exp. Med. 184:1083.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

References

  1. Dialynas, D.P. et al. (1983) J. Immunol. 131:2445.
  2. Dialynas, D.P. et al. (1983) Immunol. Rev. 74:29.
  3. Bendelac, A. (1995) Curr. Opin. Immunol. 7:367.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

Additional CD4 Products

Product Documents for Mouse CD4 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD4 Biotinylated Antibody

For research use only

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