Mouse CD45 Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # AF114
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Rat, Transgenic Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Cell Capture, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD45
Gln24-Lys425
Accession # NP_035340
Gln24-Lys425
Accession # NP_035340
Specificity
Detects mouse CD45 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse CD45 Antibody
CD45 in RAW 264.7 Mouse Cell Line.
CD45 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line (positive staining) and Neuro-2A mouse neuroblastoma cell line (negative staining) using Goat Anti-Mouse CD45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF114) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD45 in Mouse Liver.
CD45 was detected in immersion fixed paraffin-embedded sections of mouse liver using Goat Anti-Mouse CD45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF114) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Mouse CD45 by Immunocytochemistry/Immunofluorescence
The recruitment of CD45+F4/80+CD68+ macrophages was similar in the ASC-seeded DAT scaffolds that were cultured dynamically and statically for 14 days prior to implantation in the nu/nu mouse model. (A) Representative images showing CD45 (cyan), F4/80 (red), and CD68 (green) expression with DAPI counterstaining (blue) at 1-week post-implantation. Scale bars represent 100 μm. Boxed regions in the composite images are shown at higher magnification below. White arrowheads highlight CD45+F4/80+CD68+DAPI+ cells. (B) CD45+F4/80+CD68+DAPI+ cell density in the static and dynamic groups. (C) The percentage of CD45+F4/80+CD68+DAPI+ cells relative to the total CD45+DAPI+ cell population at 1, 4, and 8 weeks. *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33816453), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse CD45 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse liver
Sample: Immersion fixed paraffin-embedded sections of mouse liver
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CD45 (Catalog # 114-CD)
Sample: Recombinant Mouse CD45 (Catalog # 114-CD)
Reviewed Applications
Read 3 reviews rated 4.7 using AF114 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD45
Mouse CD45 (also known as Ly5 and leukocyte common antigen) is a 180‑220 kDa variably glycosylated member of the class 1 subtype of the protein tyrosine phosphatase family. It is synthesized as a 1291 amino acid (aa) precursor that contains a 23 aa signal sequence, a 541 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 705 aa cytoplasmic region. The ECD is coded for by exons 4‑16 of the CD45 gene. Alternate splicing of exon 4 (or A) (aa 30‑74), exon 5 (or B) (aa 75‑123) and exon 6 (or C) (aa 124‑169) define different lymphocyte populations and functional stages. Naïve T cells express exon 5 (CD45 RB), while activated T cells express neither exon 4, 5 or 6 (CD 45 RO). B cells express CD45 RABC, while resting NK cells express CD45 RA. Mouse CD45 ECD shares 60% and 44% aa sequence identity with rat and human full‑length CD45 ECD, respectively.
Long Name
Cluster of Differentiation 45
Alternate Names
CD45, LCA, PTPRC, T200 Glycoprotein
Gene Symbol
PTPRC
UniProt
Additional CD45 Products
Product Documents for Mouse CD45 Antibody
Product Specific Notices for Mouse CD45 Antibody
For research use only
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