Mouse CD5 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB115A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 53-7.3
Product Specifications
Immunogen
Mouse thymus or spleen
Specificity
Detects mouse CD5 in flow cytometry.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CD5 APC-conjugated Antibody
Detection of CD5 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD5 APC-conjugated Monoclonal Antibody (Catalog # FAB115A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse CD5 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD5
Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a Group B member of the scavenger receptor cysteine-rich protein superfamily (1). CD5 is a 67 kDa type I transmembrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is likely a ligand of the B cell differentiation antigen CD72 and is known to associate with CD6 (1). Mouse and human extracellular domains share 55% amino acid sequence indentity.
References
- Martinez, V.G., et al. (2011) Pharmacol. Rev. 63:967.
- Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
- van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
- Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.
- Luo, W. et al. (1992) J. Immunol. 148:1630.
- Lanier, L.L. et al. (1986) J. Immunol. 137:2735.
Alternate Names
CD5
Gene Symbol
CD5
Additional CD5 Products
Product Documents for Mouse CD5 APC-conjugated Antibody
Product Specific Notices for Mouse CD5 APC-conjugated Antibody
For research use only
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