Mouse CD69 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2386
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD69
Gly64-Arg199
Accession # P37217
Gly64-Arg199
Accession # P37217
Specificity
Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross‑reactivity with recombinant human CD69 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse CD69 Antibody
Detection of CD69 in mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight vs unstimulated mouse splenocytes by Flow Cytometry
Mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight (A) vs unstimulated mouse splenocytes (B) were stained with Goat Anti-Mouse CD69 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2386) and Rat Anti-Mouse CD3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4841F) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse CD69 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: - CTLL‑2 mouse cytotoxic T cell line- Mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight vs unstimulated mouse splenocytes
Sample: - CTLL‑2 mouse cytotoxic T cell line- Mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight vs unstimulated mouse splenocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CD69
Sample: Recombinant Mouse CD69
Reviewed Applications
Read 1 review rated 4 using AF2386 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD69
CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells and platelets. The C-terminal extracellular domain of mouse CD69 shares approximately 57% amino acid sequence homology with the corresponding region of human CD69.
Alternate Names
CD69, EA-1, Leu23, MLR-3, p60
Gene Symbol
CD69
UniProt
Additional CD69 Products
Product Documents for Mouse CD69 Antibody
Product Specific Notices for Mouse CD69 Antibody
For research use only
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