Mouse CD69 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB2386P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 310106
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD69
Gly64-Arg199
Accession # P37217
Gly64-Arg199
Accession # P37217
Specificity
Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD69 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse CD69 PE-conjugated Antibody
Detection of CD69 in CTLL‑2 Mouse Cell Line by Flow Cytometry.
CTLL-2 mouse cytotoxic T cell line was stained with Rat Anti-Mouse CD69 PE-conjugated Monoclonal Antibody (Catalog # FAB2386P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD69 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were (A) cultured with plate-bound anti-CD3 and anti-CD28 antibodies overnight, or (B) unstimulated, then stained with Rat Anti-Mouse CD69 PE-conjugated Monoclonal Antibody (Catalog # FAB2386P) and Rat anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on isotype control antibody (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse CD69 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: CTLL‑2 mouse cytotoxic T cell line, and Anti-CD3/CD28-activated mouse splenocytes
Sample: CTLL‑2 mouse cytotoxic T cell line, and Anti-CD3/CD28-activated mouse splenocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD69
Alternate Names
CD69, EA-1, Leu23, MLR-3, p60
Gene Symbol
CD69
UniProt
Additional CD69 Products
Product Documents for Mouse CD69 PE-conjugated Antibody
Product Specific Notices for Mouse CD69 PE-conjugated Antibody
For research use only
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