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Western blot shows lysates of mouse brain tissue and mouse thymus tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse CD90/Thy1 Monoclonal Antibody (Catalog # MAB7335) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for CD90/Thy1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of CD90/Thy1 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD90/Thy1 Monoclonal Antibody (Catalog # MAB7335) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Applications for Mouse CD90/Thy1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse splenocytes

Western Blot

0.5 µg/mL
Sample: Mouse brain tissue and mouse thymus tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD90/Thy1

CD90, also known as Thy1 (THYmocyte differentiation antigen 1) is a GPI-linked glycoprotein expressed by endothelial cells, hematopoietic stem cells, and in developing nervous tissue. Unlike human CD90, mouse CD90 is also highly expressed on T cells and is involved in T cell activation. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Alternate Names

CD90, Thy1

Entrez Gene IDs

7070 (Human); 21838 (Mouse)

Gene Symbol

THY1

UniProt

P01831

Additional CD90/Thy1 Products

Product Documents for Mouse CD90/Thy1 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Mouse CD90/Thy1 Antibody

For research use only

Citations
Reviews

Protocols
FAQs

Calculators
Flow Cytometry

Mouse CD90/Thy1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7335

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD90/Thy1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD90/Thy1 Antibody

Detection of Mouse CD90/Thy1 by Western Blot.