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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 587702

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD97v2
Gln24-His384
Accession # AAH06676

Specificity

In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) CD97 and no cross-reactivity with recombinant human (rh) CD96v2, rhCD97, or rmCD96 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse CD97 v2 Alexa Fluor® 750-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Mouse CD11c+ splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD97

CD97 is an approximately 100 kDa N-glycosylated member of the LNB-TM7 family of G protein-coupled proteins. It contains a 510 amino acid (aa) N-terminal extracellular region with four EGF-like domains and a juxtamembrane GPS motif which enables proteolytic shedding of the N-terminal extracellular domain. The alternately spliced variant 2 of CD97 exhibits a 94 aa deletion that results in loss of the third EGF-like repeat. CD97 is expressed on monocytes, macrophages, T cells, B cell subsets, dendritic cells, hematopoietic progenitor cells, and smooth muscle cells. It binds CD55/DAF, chondroitin sulfate, and Integrin although interaction with these molecules can vary between splice forms of CD97. CD97 participates in neutrophil migration, host defense, and angiogenesis. Within aa 24-384, mouse CD97 variant 2 shares 51% aa and 76% aa sequence identity with corresponding regions of human and rat CD97, respectively.

Alternate Names

CD97

Entrez Gene IDs

976 (Human); 26364 (Mouse)

Gene Symbol

ADGRE5

UniProt

Additional CD97 Products

Product Documents for Mouse CD97 v2 Alexa Fluor® 750-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD97 v2 Alexa Fluor® 750-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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