Mouse CDCP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4515
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CDCP1
Arg25-Ala667
Accession # Q5U462
Arg25-Ala667
Accession # Q5U462
Specificity
Detects mouse CDCP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human CDCP1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse CDCP1 Antibody
CDCP1 in Mouse Embryo.
CDCP1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse CDCP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4515) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing mouse brain. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Mouse CDCP1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CDCP1
Sample: Recombinant Mouse CDCP1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CDCP1
CDCP1 (CUB-domain containing protein 1; also CD318 and SIMA135) is a novel, 135 kDa cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells. It is reported that this protein is overexpressed in colon and lung cancers. It is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa 30‑667) and a phosphotyrosine site at Tyr731. When unligated, CDCP1 can be proteolytically cleaved between aa’s 270‑300. This generates an 80 kDa TM protein that may be missing the N-terminal CUB domain (aa 221‑350). Over aa’s 25‑667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.
Long Name
CUB Domain Containing Protein 1
Alternate Names
CD318, SIMA135
Gene Symbol
CDCP1
UniProt
Additional CDCP1 Products
Product Documents for Mouse CDCP1 Antibody
Product Specific Notices for Mouse CDCP1 Antibody
For research use only
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