Mouse Coagulation Factor XIV/Protein C Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4885
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Coagulation Factor XIV/Protein C
Ile19-Leu460
Accession # P33587
Ile19-Leu460
Accession # P33587
Specificity
Detects mouse Coagulation Factor XIV/Protein C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human Protein C is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse Coagulation Factor XIV/Protein C Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Coagulation Factor XIV/Protein C (Catalog # 4885-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Coagulation Factor XIV/Protein C (Catalog # 4885-SE), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Coagulation Factor XIV/Protein C (Catalog # 4885-SE)
Sample: Recombinant Mouse Coagulation Factor XIV/Protein C (Catalog # 4885-SE)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor XIV/Protein C
Protein C is a vitamin K-dependent serine protease synthesized in the liver as a single-chain precursor, which is then proteolytically processed to two disulfide-linked chains (1). The light chain consists of a Gla (gamma-carboxy-glutamate) domain and two EGF-like domains. The heavy chain consists of an activation peptide (aa 199‑212) and serine protease domain (aa 213‑449). Physiologically, Protein C is converted to the active form by thrombin, which releases the activation peptide. Protein C plays a key role in anticoagulation, cleaving factors VIIIa and Va to inactivate them. This anticoagulation activity can be enhanced by a presence of a cofactor such as protein S. In hereditary thrombophilia, Protein C deficiency is caused by a genetic mutation that affects Protein C activity. A severe recessive form may result in massive thrombosis fatal to patient.
References
- Shen, L. and Dahlbäck, B. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp.
1673.
Long Name
Vitamin K-dependent Protein C
Alternate Names
Autoprothrombin IIA, PROC, Protein C
Gene Symbol
PROC
UniProt
Additional Coagulation Factor XIV/Protein C Products
Product Documents for Mouse Coagulation Factor XIV/Protein C Antibody
Product Specific Notices for Mouse Coagulation Factor XIV/Protein C Antibody
For research use only
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