Mouse Complement Component C1ra Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7160

R&D Systems, part of Bio-Techne
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AF7160
AF7160-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Complement Component C1ra Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Complement Component C1ra
Ser17-Asn707
Accession # AAH04637

Specificity

Detects mouse Complement Component C1ra in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human C1r is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Complement Component C1ra Antibody

Detection of Mouse Complement Component C1ra antibody by Western Blot.

Detection of Mouse Complement Component C1ra by Western Blot.

Western blot shows mouse serum. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Component C1ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7160) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Complement Component C1ra at approximately 95, 60 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Complement Component C1ra Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse serum

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C1ra

C1rA (complement component 1 subcomponent r-A) is an 85-95 kDa member of the peptidase S1 family of enzymes. It is secreted primarily hepatocytes but is also known to be expressed by monocytes, fibroblasts, keratinocytes and endothelial cells. C1r forms a key component of the complement 1 complex. Complement is a general name for a group of molecules that "complement" the antimicrobial effort associated with antibodies and phagocytic cells. The C1 complex is composed of five proteins; one C1q that binds immunoglobulin, and two copies of two inactive serine proteases termed C1r and C1s. Activation of the C1 complex is the first step in a proteolytic cascade that generates multiple modulators involved in cell lysis and phagocytosis. Following C1q binding, each 85-90 kDa C1r monomer undergoes autocleavage to generate a 60 kDa and 28-34 kDa disulfide‑linked enzymatically-active heterodimer. Each C1r heterodimer now acts on a C1s counterpart to create a globally-active complex that drives the complement cascade. In contrast to human, the mouse genes for C1r and C1s have undergone duplication. The major, or liver derived, C1r is termed C1rA, while a prostate-associated C1r is called C1rB. Mouse C1rA proenzyme is 691 amino acids (aa) in length (aa 17-707) (SwissProt #: Q8CG16). It contains one CUB domain (aa 17-140), an EGF-like motif (aa 141-189), a second CUB domain (aa 192-303), two SUSHI (or CCP) repeats (aa 306-448), and a C-terminal peptidase S1 region (aa 463-704). Autocleavage occurs between Arg462:Ile463, generating a 60 kDa N-terminal subunit that is disulfide‑linked to a 30 kDa enzymatic subunit. Over aa 17-707, mouse C1rA shares 91% and 82% aa sequence identity with rat and human C1r, respectively. Full-length mouse C1rA shares 96% aa sequence identity with mouse C1rB, with virtually all differences arising in the enzymatic, or small subunit.

Alternate Names

C1ra

Entrez Gene IDs

50909 (Mouse)

Gene Symbol

C1RA

UniProt

AAH04637

Additional Complement Component C1ra Products

Product Documents for Mouse Complement Component C1ra Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse Complement Component C1ra Antibody

For research use only

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