J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Mouse Complement Component C5a R1 Monoclonal Antibody (Catalog # MAB6467, filled histogram) or isotype control antibody (MAB0061), open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (F0113).

Applications for Mouse Complement Component C5aR1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C5aR1

C5a R1, also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5a R1 binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5a R1/C5a interactions are protective during allergen sensitization. Mouse C5a R1 shares 66% and 77% amino acid sequence identity with human and rat C5a R, respectively.

Long Name

Complement Component C5a Receptor 1

Alternate Names

C5AR1, CD88, Complement Component C5a R1

Entrez Gene IDs

728 (Human); 12273 (Mouse)

Gene Symbol

C5AR1

UniProt

P30993

Additional Complement Component C5aR1 Products

Product Documents for Mouse Complement Component C5aR1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Mouse Complement Component C5aR1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Complement Component C5aR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6467

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Complement Component C5aR1 Antibody

Detection of Complement Component C5a R1 in J774A.1 Human Cell Line by Flow Cytometry.